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Benchling Inc pten guide rna
Pten Guide Rna, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pten+guide+rna/pten+guide+rna/pm37772705-185-9-20
Average 90 stars, based on 1 article reviews
pten guide rna - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

CRISPR:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Selection:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Control:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Western Blot:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Electroporation:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Concentration Assay:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Next-Generation Sequencing:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Sequencing:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Mutagenesis:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Immunohistochemistry:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Staining:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

MANN-WHITNEY:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Immunofluorescence:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Competitive Binding Assay:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.

Cell Culture:

Article Title: Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR.
Article Snippet: Regions of interest included the area targeted by the PTEN guide RNA and the top off-target regions as predicted by Benchling.



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Genecopoeia single-guide rna (sgrna) plasmids targeting mouse pten (mcp227985-cg04-3-10)
Single Guide Rna (Sgrna) Plasmids Targeting Mouse Pten (Mcp227985 Cg04 3 10), supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pten Guide Rna, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pten+guide+rna/pten+guide+rna/pm37772705-185-9-20
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Addgene inc pten guide rna
Establishment of an A2.DR1 MHC-humanized glioma model. A, Experimental overview. P0 A2.DR1 pups were electroporated with plasmids encoding for CRISPR-Cas9 and 3 guide <t>RNAs</t> (gRNA) targeting p53, Nf1, and <t>Pten.</t> Growing tumors were excised, passaged in NSG mice, and used to generate a cell line that was reimplanted into adult A2.DR1 mice. MRI and H&E histology are shown for respective tumor stages. B, Pie chart depicting the proportions of mutations that are shared by the parental tumor and ex vivo passaged cell line or that are newly acquired after in vitro passage. C, Verification of the CRISPR-mediated functional knockout of p53 in the A2.DR1 glioma cell line. D, Representative immunofluorescence images of A2.DR1 glioma orthotopically injected into the brain of adult A2.DR1 mice, 20 days after injection. E, MHC expression of the A2.DR1 glioma cell line, incubated for 24 hours with recombinant murine IFNγ. FMO, fluorescence minus one control. F, Immunofluorescence image of CicR215W overexpressing A2.DR1 glioma cells.
Pten Guide Rna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pten+guide+rna/pten+guide+rna/pmc09401455-46-11-18
Average 90 stars, based on 1 article reviews
pten guide rna - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Establishment of an A2.DR1 MHC-humanized glioma model. A, Experimental overview. P0 A2.DR1 pups were electroporated with plasmids encoding for CRISPR-Cas9 and 3 guide RNAs (gRNA) targeting p53, Nf1, and Pten. Growing tumors were excised, passaged in NSG mice, and used to generate a cell line that was reimplanted into adult A2.DR1 mice. MRI and H&E histology are shown for respective tumor stages. B, Pie chart depicting the proportions of mutations that are shared by the parental tumor and ex vivo passaged cell line or that are newly acquired after in vitro passage. C, Verification of the CRISPR-mediated functional knockout of p53 in the A2.DR1 glioma cell line. D, Representative immunofluorescence images of A2.DR1 glioma orthotopically injected into the brain of adult A2.DR1 mice, 20 days after injection. E, MHC expression of the A2.DR1 glioma cell line, incubated for 24 hours with recombinant murine IFNγ. FMO, fluorescence minus one control. F, Immunofluorescence image of CicR215W overexpressing A2.DR1 glioma cells.

Journal: Clinical Cancer Research

Article Title: T-cell Receptor Therapy Targeting Mutant Capicua Transcriptional Repressor in Experimental Gliomas

doi: 10.1158/1078-0432.CCR-21-1881

Figure Lengend Snippet: Establishment of an A2.DR1 MHC-humanized glioma model. A, Experimental overview. P0 A2.DR1 pups were electroporated with plasmids encoding for CRISPR-Cas9 and 3 guide RNAs (gRNA) targeting p53, Nf1, and Pten. Growing tumors were excised, passaged in NSG mice, and used to generate a cell line that was reimplanted into adult A2.DR1 mice. MRI and H&E histology are shown for respective tumor stages. B, Pie chart depicting the proportions of mutations that are shared by the parental tumor and ex vivo passaged cell line or that are newly acquired after in vitro passage. C, Verification of the CRISPR-mediated functional knockout of p53 in the A2.DR1 glioma cell line. D, Representative immunofluorescence images of A2.DR1 glioma orthotopically injected into the brain of adult A2.DR1 mice, 20 days after injection. E, MHC expression of the A2.DR1 glioma cell line, incubated for 24 hours with recombinant murine IFNγ. FMO, fluorescence minus one control. F, Immunofluorescence image of CicR215W overexpressing A2.DR1 glioma cells.

Article Snippet: Briefly, A2.DR1 P0 pups were electroporated with p53, NF1 , and Pten guide RNAs cloned into pX330 plasmids (Addgene).

Techniques: CRISPR, Ex Vivo, In Vitro, Functional Assay, Knock-Out, Immunofluorescence, Injection, Expressing, Incubation, Recombinant, Fluorescence, Control